Fig. 10: Identification of PKC and PKA phosphorylation sites in L. salmonis Glps. | Communications Biology

Fig. 10: Identification of PKC and PKA phosphorylation sites in L. salmonis Glps.

From: Lineage-level divergence of copepod glycerol transporters and the emergence of isoform-specific trafficking regulation

Fig. 10

A Amino acid alignment of the N-terminus and intracellular loop B of L. salmonis Glp1_v1, Glp1_v2, Glp2, Glp3_v1 and Glp3_v2. Asterisks and colons under the alignment indicate fully conserved residues and conservation between groups of strongly similar properties, respectively. Functional and non-functional phosphorylation sites by PKC (red) or PKA (blue) are indicated. B, C, E, F Pf of oocytes injected with water or expressing wild-type Glps or mutant channels at the putative PKC and PKA phosphorylation residues and treated with PMA and/or IBMX/FSK. Data are the mean ± SEM (number of oocytes indicated on top of each bar). *p < 0.05; ***p < 0.001, statistically different (one-way ANOVA) as indicated in brackets. D, G Representative immunoblot of total membrane protein extracts from oocytes injected with each construct showing equivalent expression. Molecular mass markers (kDa) are on the left.

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