Fig. 2: Antibody specificity against L. salmonis Glps. | Communications Biology

Fig. 2: Antibody specificity against L. salmonis Glps.

From: Lineage-level divergence of copepod glycerol transporters and the emergence of isoform-specific trafficking regulation

Fig. 2

A–E Western blot of total membranes of X. laevis oocytes injected with water or expressing different L. salmonis aquaporins. Membranes were probed with paralog-specific antibodies against Glp1_v1, Glp1_v2, Glp2, Glp3_v1 or Glp3_v2 as indicated. Note that none of the antisera showed cross-reactivity with another aquaporin. F–J Detection of Glps in protein extracts from adult whole female and male L. salmonis. The blot on the right in each panel was incubated with the corresponding primary antibody preadsorbed with the antigenic peptide. The asterisks in G and I indicate the cross-reaction of the Glp1_v2 and Glp3_v1 antisera, respectively, with a polypeptide of ~55 kDa in males or females. In all panels, the aquaporin monomers are indicated with an arrow, whereas molecular mass markers (kDa) are on the left.

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