Fig. 8: Regulation of L. salmonis Glp intracellullar trafficking in X. laevis oocytes. | Communications Biology

Fig. 8: Regulation of L. salmonis Glp intracellullar trafficking in X. laevis oocytes.

From: Lineage-level divergence of copepod glycerol transporters and the emergence of isoform-specific trafficking regulation

Fig. 8

A, D, G, J, M Representative immunofluorescence photomicrographs of paraffin sections of water and Glp-injected oocytes, the latter treated with the drug vehicle (DMSO, control), PMA or IBMX plus FSK. Scale bars, 25 µm. B, E, H, K, N Percentage of each Glp in the oocyte plasma membrane (PM) after each treatment determined by image analysis. Data are the mean ± SEM (n = 6 oocytes) were statistically analyzed by one-way ANOVA, followed by the Dunnett’s multiple comparison test. ***p < 0.001, with respect to DMSO-treated oocytes. C, F, I, L, O Representative immunoblots of Glp1_v1, Glp1_v2, Glp2, Glp3_v1 or Glp3_v2 in total membrane and plasma membrane purifications of oocytes expressing each Glp and treated with DMSO, PMA or IBMX/FSK. In all panels, the molecular mass markers (kDa) are on the right.

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