Fig. 5: Correlation between interaction with heme and sequence features of the variable regions of therapeutic Abs. | Communications Biology

Fig. 5: Correlation between interaction with heme and sequence features of the variable regions of therapeutic Abs.

From: Interaction of clinical-stage antibodies with heme predicts their physiochemical and binding qualities

Fig. 5

The binding intensity to immobilized cofactors (heme and FA, Fig. 1b) and the degree of heme-induced antigen polyreactivity (Fig. 3a) were correlated with the sequence characteristics of heavy and light immunoglobulin chain variable regions of the studied therapeutic Abs. The heat map shows the values of the correlation coefficient (ρ) obtained after Spearman’s rank-order analysis of the correlation of data from the binding assays with the number of amino acid replacements in heavy chain variable region; the lengths of CDRH3, CDRH2, and CDRL3; or the number of particular amino acid residues that are present in CDRH3, CDRH2, and CDRL3. The red color in the heat map signifies the positive value of the correlation coefficient, the blue-negative. The summary of statistical significance (P values) is depicted on the right panels.

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