Fig. 6: Molecular features of antigen-binding sites of selected heme binding and heme sensitive Abs. | Communications Biology

Fig. 6: Molecular features of antigen-binding sites of selected heme binding and heme sensitive Abs.

From: Interaction of clinical-stage antibodies with heme predicts their physiochemical and binding qualities

Fig. 6

(Left column) Coulomb’s electrostatic potential of antigen-binding sites of selected Abs. The distribution of the electrostatic charges (blue for positive charge and red for a negative charge) was colored using UCSF Chimera software. The selected polyreactive Abs demonstrated the most pronounced binding to immobilized heme and/or heme-induced polyreactivity. (Right column) Structural models of antigen-binding sites depicting the surface exposed Tyr residues. Gray: variable domain of light chain; blue: variable domain of heavy chain, orange: tyrosine residues. The structural models were visualized by using UCSF Chimera software. All structural models of the variable immunoglobulin regions were built by ROSSIE online server.

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