Fig. 7: Analysis of the correlations among biophysical developability measurables and cofactors interactions parameters.

a Matrix depicting the correlation of 12 different Ab developability parameters with Ab-binding intensity to immobilized cofactors (heme and FA—binding), the degree of heme-induced antigen polyreactivity (heme-poly), and Ab interaction with heme in solution (λ shift, ΔA and ABTS). The heat map shows the values of correlation coefficient (ρ) obtained after Spearman’s rank-order analysis of the correlation of data. The red color in the heat map signifies positive value of correlation coefficient, the blue—negative. The summary of statistical significance (P values) is depicted on the right panel. Data related to the biophysical developability measurables of the antibodies were obtained from the study of Jain et al.³². b Graphical output of the Principal Component Analysis where biophysical developability measurables (in black) and cofactors interactions parameters (in green) are represented as vectors in a plane defined by dimension 1 (horizontal axis) and dimension 2 (vertical axis), explaining 25.7% and 15.3% of the variability of the dataset, respectively. Projections of the vectors on each axis account for the quality of representation of the parameters by dimensions 1 and 2. Positively correlated parameters are grouped; negatively correlated parameters are represented in opposite quadrants and non-correlated parameters are shown by orthogonal vectors.