Table 2 Antibodies and fluorophores.
From: An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
Antibody & fluorophore | Clone | Vendor – Catalog number | Host | Dilution | Time | Temp. |
|---|---|---|---|---|---|---|
E-cadherin, direct conjugate (AlexaFluor-488) | 24E10 | Cell Signaling Technologies – 3199S | Rabbit | 1:100 | O/N | 4 °C |
Vimentin, direct conjugate (AlexaFluor-555) | D21H3 | Cell Signaling Technologies – 9855S | Rabbit | 1:50 | O/N | 4 °C |
DAPI | NA | ThermoFisher | NA | 1:100 | 15 min | RT |
NOS2, direct conjugate (AlexaFluor-568) | EPR16635 | AbCam – 209595 | Rabbit | 1:100 | 1 h or O/N | RT 4 °C |
COX2, direct conjugate (AlexaFluor-488) | D5H5 | Cell Signaling Technologies – 13596S | Rabbit | 1:100 | 1 h or O/N | RT 4 °C |
HIF1α, direct conjugate (AlexaFluor-647) | EPR16897 | AbCam – 208420 | Rabbit | 1:100 | O/N | 4 °C |
ATP5A1 (Mitochondrial ATPase, Primary) | NA | AbClonal – A5884 | Rabbit | 1:100 | O/N | 4 °C |
(Anti-rabbit CF-640R secondary for ATPase) | NA | Biotium – 20178 | Donkey | 1:100 | O/N | 4 °C |
α-Smooth Muscle Actin, direct conjugate (eFluor-488), eBioscience | 1A4 | ThermoFisher – 41-9760-82 | Mouse | 1:400 | O/N | 4 °C |
