Fig. 6: Heavy metal accumulation in yeasts in which the crown-of-thorns starfish (COTS), Acanthaster planci, divalent metal transporter (ApDMT) and DMT-related proteins (ApDMTRP) were expressed.
From: Divalent metal transporter-related protein restricts animals to marine habitats
Wild-type or metal uptake-deficient strains of yeasts were transformed with the expression vector pDR195 containing ApDMT or ApDMTRP or empty vector (EV), and exposed to media containing FeCl3 (a, b), MnSO4 (c, d), ZnCl2 (e, f), CdCl2 (g), PbCl2 (h), or CuCl2 (i). The vertical axis shows the intracellular concentration measured by ICP-MS normalized by yeast optical density (OD600). The horizontal axis shows the names of expressed genes/yeast strains. Each bar represents the mean ± SEM (n = 3). One or two asterisks on the bar represent statistical significance by one way ANOVA with Tukey’s post-hoc HSD test at p < 0.01(**) or p < 0.05(*). DY, wild-type strain of DEY1453 and ZHY3; DEY, Fe-uptake-deficient yeast strain DEY1453; ZHY, Zn-uptake-deficient yeast strain ZHY3; BY4743, wild-type strain of single mutant strain HomDip-YOL122C lacking SMF1; smf1, Mn-uptake-deficient yeast strain HomDip-YOL122C lacking SMF1. Dot plots shows individual data points. Dot plots data are in Supplementary Data 2.
