Fig. 1: Loss of COMMD4 results in genomic instability.
From: COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks
a, b U20S cells were transfected with COMMD4 esiRNA (#1) and/or siRNA #2, siRNA #3 and siRNA #4 to deplete COMMD4 protein levels and treated with varying doses of IR or CPT. A clonogenic assay was subsequently carried out to determine the sensitivity of COMMD4-depleted cells to these agents. AT5; cells from an A-T patient. Asterix (*) denotes p < 0.05. Error bars represent mean ± SD from three independent experiments. c Immunofluorescence on control and COMMD4-depleted U2OS cells treated with 6 Gy of IR followed by recovery at the time points shown and stained with γH2AX. d γH2AX foci in control and COMMD4-depleted cells using the OMX super-resolution microscope. e Plot of γH2AX foci numbers for c and d. f Immunofluorescence experiment demonstrating MDC1 and hSSB1 foci formation in control and COMMD4-depleted cells before and 2 h after irradiation. g, h Immunofluorescence experiment demonstrating RPA and Rad51 foci formation respectively in control and COMMD4-depleted cells before and after irradiation. DAPI shows the nucleus. ns not significant where p > 0.05, *p < 0.05 and **p < 0.005. Error bars represent mean ± SD from three independent experiments where 50 cells were quantified per condition. Scale bar denotes 5 μm.
