Fig. 4: FGL2 induced pro-repair macrophages polarization through binding to CD32B. | Communications Biology

Fig. 4: FGL2 induced pro-repair macrophages polarization through binding to CD32B.

From: Regulatory T cells induce polarization of pro-repair macrophages by secreting sFGL2 into the endometriotic milieu

Fig. 4

a Heat map of differentially expressed genes in macrophages treated with PBS (crtl) or rFGL2. b Enriched GO analysis of the top ten GO terms changed in macrophages treated with rFGL2. c Heat map of representative pro-inflammatory and pro-repair related genes differentially expressed by macrophages treated with rFGL2. D-E, Flow cytometric analysis (d), qRT-PCR and ELISA analysis (e) for the expression of pro-inflammatory (CD86, NOS2, IL-12p70) and pro-repair (CD163, CD206, Arg1, IL-10) macrophages markers in THP-1 and U937 cells treated with rFGL2 or PBS. f CD86, HLA-DR, IL-6, and IL-12p70 expression in macrophages treated with rFGL2 or pretreated with anti-CD32B antibody 2 h before adding rFGL2 (0.5 µg per ml). g CD163, CD206, TGF-β, and IL-10 expression in macrophages treated with rFGL2 or pretreated with anti-CD32B antibody 2 h before adding rFGL2 (0.5 µg per ml). Data represent the mean value ± SEM of five individual experiments (n = 5).

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