Fig. 4: Cryo-EM study of heme-stabilized α-Syn oligomers. | Communications Biology

Fig. 4: Cryo-EM study of heme-stabilized α-Syn oligomers.

From: Conformational distortion in a fibril-forming oligomer arrests alpha-Synuclein fibrillation and minimizes its toxic effects

Fig. 4

a–c Heme treatment on monomeric α-Syn for 24 h (preincubation). a Raw micrograph showing uniformly distributed small oligomers (green squares, oligomers1). b I–III Reference-free 2D class averaging analyses were done by Xmipp, RELION and EMAN2 (refer Methods). c Cryo-EM density map of α-Syn oligomers1. d, e Heme treatment performed after fibril formation has initiated, i.e., after 48 h of aggregation (postincubation). d Raw micrograph showing distribution of small oligomers (oligomers2) similar to oligomers1, as well as semi-annular-shaped oligomers and fibrils are marked by green squares, orange squares and a yellow arrow, respectively. e I–III Reference-free 2D class averaging analyses were done by Xmipp, RELION and EMAN2. f The density map of α-Syn oligomers2. In panels c and f, the dotted black arrow indicates the kink between the head and base of the oligomers.

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