Fig. 2: Transcriptional profile of intrinsic mOSE stemness.
From: Transcriptional heterogeneity of stemness phenotypes in the ovarian epithelium
a Plot showing the distribution of differentially expressed genes in mOSE cells cultured as spheroids for 14 days relative to a monolayer (n = 3). Each point corresponds to a single gene. Selected genes related to stemness and/or the EMT are highlighted on the plot. Dashed lines correspond to significance criteria (absolute log fold change >0.5, p < 0.05). b Boxplots showing the expression values of putative mOSE stemness markers in mOSE cells cultured in a monolayer (Control) or as spheroids (n = 3). Boxplots show median value (horizontal black line), estimated 25th and 75th percentiles, and whiskers represent 1.5 times the interquartile range c GSEA results for selected gene sets enriched in differentially expressed genes in mOSE spheroids. All gene sets are significantly enriched (p < 0.05) and normalized enrichment scores (NES) are shown. d Inferred pathway activity in monolayer- and spheroid-cultured mOSE cells. Linear models were used for statistical testing for b and d. *p < 0.05, **p < 0.01, ***p < 0.001.
