Fig. 3: TGFB1 promotes a distinct stemness phenotype.
From: Transcriptional heterogeneity of stemness phenotypes in the ovarian epithelium
a Plot showing the distribution of differentially expressed genes in monolayers of mOSE cells treated with TGFB1 compared to untreated samples (n = 3). Each point corresponds to a single gene. Selected genes related to stemness and/or the EMT are highlighted on the plot. Dashed lines correspond to significance criteria (absolute log fold change >0.5, p < 0.05). b GSEA results for selected gene sets enriched in differentially expressed genes following TGFB1 treatment. All gene sets are significantly enriched (p < 0.05) and NES values are shown. c Inferred pathway activity in untreated and TGFB1-treated mOSE cells. P-values were computed from the t statistic of a linear regression model and were adjusted using the Benjamini–Hochberg false discovery rate (FDR) method. d Plot comparing log fold-change values for spheroid-cultured and TGFB1-treated mOSE. Dashed lines correspond to fold change cutoffs used to assess significance. e Plots showing gene sets enriched in commonly up- or downregulated genes following both TGFB1 treatment and spheroid culture. P-values were calculated using a Fisher exact test and were adjusted using the Bejamini–Hochberg FDR method.
