Fig. 5: Brca1 deletion in vivo promotes increased label retention.
From: Transcriptional heterogeneity of stemness phenotypes in the ovarian epithelium
a Primary (left; n = 3) and secondary (middle; n = 5) sphere forming capacity of mOSE cells following Brca1 deletion by infection with Ad-Cre (Brca1 KO). Cells infected with Ad-GFP were used as a control (Ctrl). Data points represent the average number of spheres per 4 fields of view for each replicate. Right: Phase contrast images of control (OSE from PBS-injected mice) and BRCA KO spheroids. Spheroids were cultured for 14 days. Scale bar = 100 μm. b Immunohistochemical staining of ovaries following intrabursal injection of PBS or Ad-Cre. Staining shows BRCA1 (red) and the YFP (green) reporter activated upon delivery of Cre recombinase. Nuclei are stained with DAPI (blue). Scale bar = 25 μm. c BrdU label retention (red) and YFP (green) signal in ovaries following intrabursal injection of either PBS or Ad-Cre. Scale bar = 100 μm, inlet scale bar = 25 μm. d Quantification of BrdU+ OSE cells in ovaries (n = 4 ovaries from independent mice). All boxplots show median value (horizontal black line), estimated 25th and 75th percentiles, and whiskers represent 1.5 times the interquartile range.
