Fig. 1: Design of reporters to measure stress response of non-replicating STM. | Communications Biology

Fig. 1: Design of reporters to measure stress response of non-replicating STM.

From: The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress

Fig. 1

A For detection of NR intracellular STM, the EM7 promoter of dual FP reporters for msrA, trxA, or htrA was replaced by the tet-ON cassette to enable controlled induction of dsred expression by AHT. B Addition of AHT to growing cultures of STM induced DsRed synthesis. Before infection of macrophages, AHT was removed by centrifugation and washing. Infection was performed in absence of AHT. After infection, intracellular R STM dilute cellular DsRed levels. NR intracellular STM maintain cellular DsRed levels and were detected as DsRed-positive events. In addition, the msrA-induced sfGFP intensity provides information about response to stress imposed by the host cell.

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