Fig. 3: Non-replicating SPI2-T3SS or SR mutant strains also show lower stress response compared to respective subpopulation. | Communications Biology

Fig. 3: Non-replicating SPI2-T3SS or SR mutant strains also show lower stress response compared to respective subpopulation.

From: The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress

Fig. 3

STM WT, ΔsseF, ΔsifA, ΔssaV, and ΔdksA strains harboring AHT-inducible (NR), or constitutive (entire population, EP) dual-fluorescence reporter for msrA were grown o/n in LB medium. AHT was present for AHT-inducible reporters and removed before infection. RAW264.7 macrophages were infected, lysed 24 h p.i., liberated STM were recovered, fixed, and subjected to FC analyses. As negative control, a dual FP reporter plasmid with sfgfp inactivated by a frameshift was used. A The x-median represents the msrA-induced sfGFP signal of the DsRed-positive intracellular bacterial population at 24 h p.i. Means and standard deviations of one representative experiment are shown. Statistical analysis was performed as described for Fig. 2 and is indicated for NR vs. EP for each strain. B The depicted slopes represent the sfGFP intensity increase over the time of infection. C Representative histograms corresponding to A are shown.

Back to article page