Fig. 5: Cefotaxime-treated non-replicating STM WT show slight induction of stress response. | Communications Biology

Fig. 5: Cefotaxime-treated non-replicating STM WT show slight induction of stress response.

From: The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress

Fig. 5

STM WT harboring AHT-inducible (NR) or constitutive (EP) dual-fluorescence reporter for msrA was grown o/n in LB medium, in the presence of AHT or the NR reporter. Before infection, AHT was removed. RAW264.7 macrophages were infected, cefotaxime was added to the cells at 10 h p.i. if indicated, and cells were lysed at 24 h p.i. Liberated STM were recovered, fixed and subjected to FC. As negative control, a dual FP reporter plasmid with sfgfp inactivated by a frameshift was used. The x-median (A) represents the PmsrA-induced sfGFP signal of the DsRed-positive intracellular bacterial population, and B shows a representative histogram. Means and standard deviations of one representative experiment are shown. Statistical analysis was performed as described for Fig. 2.

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