Fig. 6: Stress response of non-replicating STM WT in primary human macrophages. | Communications Biology

Fig. 6: Stress response of non-replicating STM WT in primary human macrophages.

From: The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress

Fig. 6

STM WT harboring AHT-inducible (NR) or constitutive (EP) dual-fluorescence reporter for msrA was grown o/n in LB medium in the presence of AHT if necessary. Before infection, AHT was removed and murine RAW264.7 or human primary macrophages were infected. Host cells were lysed 24 h p.i., liberated STM were recovered, fixed, and subjected to FC. As negative control, a dual FP reporter plasmid with sfgfp inactivated by a frameshift was used. A Detection of intracellular NR STM in human macrophages. B Comparison of msrA induction within murine and human macrophages. The x-median represents the msrA-induced sfGFP signal of DsRed-positive intracellular bacteria. Means and standard deviations of one representative experiment are shown. C STM WT only shows a NR subpopulation when plotting the bacterial population against their AHT-induced DsRed and msrA-induced sfGFP intensity. Statistical analysis was performed as described for Fig. 2.

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