Fig. 1: Establishment of a DENV DIP production cell line using HEK 293T cells.
From: Dengue virus-free defective interfering particles have potent and broad anti-dengue virus activity
A A lentiviral vector expressing the DENV-2 NS proteins and EGFP. B A lentiviral vectors that expresses the DI-290 RNA as an mRNA that is processed by the HDVr into authentic DI-290 RNA and expresses CFP. C A retroviral vector that expresses DENV-2 S proteins and mCherry. D The transduction of HEK 293T cells can be selected by FACS for triple-positive cells expressing EGFP, mCherry and CFP. E Western blot of HEK-DI-290-ORF cells using antibodies to DENV NS5, NS3, C and E proteins. The relative protein loading in each lane is indicated by a western blot for β-tubulin. F Confocal fluorescence microscopy of HEK 293T, DENV-2 infected HEK 293T, and HEK-DI-290-ORF cells stained with antibodies to DENV E, C, and NS3 (magenta). Cells were counter stained with DAPI (blue). The white bar in the image represents 10 µm. mol. wt. is molecular weight.
