Fig. 4: MFN2 is required for basal mitochondrial respiration and mtROS production during bacterial infection. | Communications Biology

Fig. 4: MFN2 is required for basal mitochondrial respiration and mtROS production during bacterial infection.

From: Mitofusin-2 boosts innate immunity through the maintenance of aerobic glycolysis and activation of xenophagy in mice

Fig. 4

a, b Respiratory profile (OCR) (a) and their respiratory parameters; Basal respiration (Basal resp) and ATP production OCR (ATP prod) (b) in Mfn2 WT and Mfn2 CKO PMs infected with Mtb (MOI 5) for 18 h. c qPCR analysis of Ndufab1 in Mfn2 WT and Mfn2 CKO PMs infected with Mtb (MOI 5) for indicated time (n = 3). d Western blot analysis of OXPHOS complexes in Mfn2 WT and Mfn2 CKO BMDMs infected with Mtb (MOI 5) as indicated. e Densitometry analysis of NDUFA9 western blot represented in (d) (n = 3). f Representative MitoSOX (red) or DAPI (for nuclei; blue)-stained images. BMDMs from Mfn2 WT or Mfn2 CKO mice were infected with Mtb (MOI 5 for 4 h) and visualized by confocal microscopy. Scale bars, 50 μm. g Average mean fluorescence intensities (MFI) of MitoSOX. Data are presented as mean ± SD (c) or mean ± SEM (b, e, g) and are representative of three independent experiments. Two-tailed Student’s t test is used to calculate the significance (b, c, e, g). Un, uninfected.

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