Fig. 4: ApoJ silencing reduced SOAT2 expression and SOAT enzymatic activity. | Communications Biology

Fig. 4: ApoJ silencing reduced SOAT2 expression and SOAT enzymatic activity.

From: Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses

Fig. 4

Huh7.5 cells bearing Luc or ApoJ shRNA were established to examine a SOAT2 mRNA (n = 3); b SOAT2 protein expression (representative image in left panel; SOAT2 intensity in right panel, n = 4) and; c SOAT enzymatic activity in the presence of FFAs or HCV infection at MOI = 0.01 for 6 days with and without TMP153 treatment; and d, e image visualization of NBD-CE under confocal microscopy observation. The data were presented as the mean ± SEM (n ≧ 6), and asterisks indicate statistical significance (p < 0.05) between the control and ApoJ knockdown cells. Scale bar, 5 μm.

Back to article page