Fig. 5: ApoJ co-localized with SOAT2 by recognizing the IDR region. | Communications Biology

Fig. 5: ApoJ co-localized with SOAT2 by recognizing the IDR region.

From: Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses

Fig. 5

The IDR regions of SOAT1 (UniProt ID: P35610, a) and SOAT2 (UniProt ID: O75908, b) were predicted by D2P2 (http://d2p2.pro/search) and IUPred2A (https://iupred2a.elte.hu/) algorithms, respectively. Huh7 cells were transfected with SOAT1-DsRed (c, the left panels), IDR-truncated SOAT1-DsRed (ΔSOAT1-DsRed, c, the right panels); SOAT2-DsRed (d, the left panels), and IDR-truncated SOAT2-DsRed (ΔSOAT2-DsRed, d the right panels), followed by administration of PA (100 μM) or OA (400 μM) for 24 h, and visualized with confocal microscopy. The fluorescence intensity was analysed by FVW31S software. The Mander’s coefficient was analysed by ImageJ with the Coloc2 plugin from ImageJ/Fiji shown above. Scale bar, 5 μm. Huh7 cells were transfected with plasmids encoding SOAT2-DsRed (e) or ΔSOAT2-DsRed (f). The cell lysates were immunoprecipitated with antibody against ApoJ and examined by western blot analysis recognizing DsRed and apoJ.

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