Fig. 4: Galactose sensitises cells to loss of mitochondrial genes and promotes loss of genes involved in energy-consuming processes. | Communications Biology

Fig. 4: Galactose sensitises cells to loss of mitochondrial genes and promotes loss of genes involved in energy-consuming processes.

From: Genome-wide CRISPR/Cas9 deletion screen defines mitochondrial gene essentiality and identifies routes for tumour cell viability in hypoxia

Fig. 4

a Schematic diagram shows which datasets were compared in our analysis (normoxia-galactose vs. normoxia-glucose). Sequencing reads from triplicate incubations were analysed by the MAGeCK analysis platform, and relative sgRNA abundances were calculated between experimental conditions. b Pie charts show number of mitochondrial genes among genes with significantly depleted or enriched sgRNAs from cells in normoxia-galactose compared to normoxia-glucose. c Panel shows all mitochondrial genes with significantly depleted sgRNAs from (b). Genes significantly depleted in normoxia-glucose (plasmid vs. library) are highlighted in brown. d Chart shows relative growth of U2OS cells incubated in 25 mM normoxia-glucose or normoxia-galactose, and left untreated (NT) or treated with 31.25 nM rotenone for 72 h. n = 3; mean ± S.D.; ***p < 0.001. e Chart shows overrepresentation analysis of all genes with significantly enriched sgRNAs from (a). f Schematic diagram shows node of selected interacting genes with significantly enriched sgRNAs from (a) involved in mRNA processing. g Chart shows all genes with significantly enriched gRNAs (robust rank aggregation (RRA) score, positive) from (a). Hexose metabolism genes highlighted in blue.

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