Fig. 3: OLIG2+ stem cells decline more rapidly in M-Smo compared to G-Smo tumors. | Communications Biology

Fig. 3: OLIG2+ stem cells decline more rapidly in M-Smo compared to G-Smo tumors.

From: Cryptic developmental events determine medulloblastoma radiosensitivity and cellular heterogeneity without altering transcriptomic profile

Fig. 3

a Representative higher magnification images of OLIG2/SOX10 IHC on a G-Smo tumor, showing OLIG2+/SOX10+ oligodendrocytes (arrowheads highlight examples) and OLIG2+/SOX10− tumor stem cells. b Representative lower magnification images of OLIG2/SOX10 IHC on sagittal sections from mice of the indicated age, genotype, and treatment. Arrowheads highlight a perivascular region. Scale bars = 100 µm in a and 1 mm in b. c Quantitative comparison of OLIG2+/SOX10− populations in G-Smo and M-Smo tumors. d Western blots of replicate G-Smo and M-Smo tumors, showing pOLIG2 and β-Actin. Quantification shows pOLIG2 signal, normalized to β-Actin, expressed as fold change relative to mean G-Smo value. In graphs in c and d, Student’s t-test was used to make pairwise comparisons, dots represent values for individual replicates, bars indicate the means and whiskers indicate the SEM.

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