Fig. 6: M-Smo tumors show macrophage and microglial populations not present in G-Smo tumors. | Communications Biology

Fig. 6: M-Smo tumors show macrophage and microglial populations not present in G-Smo tumors.

From: Cryptic developmental events determine medulloblastoma radiosensitivity and cellular heterogeneity without altering transcriptomic profile

Fig. 6

a UMAP showing myeloid-like cells from G-Smo, M-Smo, and P7 WT, with clusters color-coded and circled. b Feature plot on UMAP from a, showing expression of the pan-macrophage and microglial marker C1qb and the choroid plexus epithelial markers Cnn3 and Meis1. Cluster 4 does not show C1qb expression. c–f Feature plots on UMAP from a, showing expression of c Cluster M0 markers Cx3cr1 and Sparc, d Cluster M1 markers Igf1, Mrc1, and wfdc17, or e Cluster M2 markers Ccr2, Cd74, H2-Ea-ps, and Il1b f Cluster M3 markers Mrc1 and Cd163. g genotype color-coded on the UMAP from a. Circles indicate the positions of the clusters. h Comparison of the indicated cluster populations in G-Smo, M-Smo, and P7 WT. i Violin plots showing the number of cells expressing the indicated level of Mif in each genotype. j IHC for p4EBP1 in representative G-Smo and M-Smo tumors at low and higher magnification, and quantification of p4EBP1+ fractions of tumor cells in each genotype. Scale bars = 2 mm in low magnification images and 200 µm in higher magnification images. Student’s test was used in h, j and two-proportions z-test in i. In graphs in h and j, dots represent values for individual replicates, bars indicate the means and whiskers indicate the SEM.

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