Fig. 6: Histopathological, immunohistochemical and biochemical characteristics of regenerated neo-corneas at 12 months post-implantation with CLP-PEG-MPC implants compared to CLP-PEG implants. | Communications Biology

Fig. 6: Histopathological, immunohistochemical and biochemical characteristics of regenerated neo-corneas at 12 months post-implantation with CLP-PEG-MPC implants compared to CLP-PEG implants.

From: Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs

Fig. 6

a Corneal epithelial hyperplasia was noticeably higher in the CLP-PEG only implants (black circles) compared to CLP-PEG-MPC implants (pink squares), while neovascularization was not markedly different. However, neither was statistically significant by the Mann–Whitney U test. b Mean cell counts normalized to the contralateral control eye for myofibroblast α-smooth muscle actin, blood vessel marker CD31, lymphatic vessel marker LYVE1, and the myeloid cell marker CD172a show no significant differences between CLP-PEG-MPC (pink squares) and CLP-PEG (black circles). Statistical analysis by unpaired, two-way t-test were performed with statistical significance set at p≤0.05. c Collagen content analysis of the central cornea in CLP-PEG-MPC (pink squares), CLP-PEG (black circles) and unoperated (aqua triangles) corneas. Statistical analysis of collagen by two-way ANOVA with Tukey’s multiple comparisons test. Data are displayed using the mean. *Unoperated vs CLP-PEG, p<0.05. †Unoperated vs. CLP-PEG-MPC, p≤0.05. ‡ CLP-PEG vs. CLP-PEG-MPC, p≤0.05. For all charts the unit of analysis is the eye: CLP-PEG n=4, CLP-PEG-MPC n=4, unoperated n=8.

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