Fig. 3: Regulatory signature of CD8+ T cells stimulated via TCR without CD28 costimulation. | Communications Biology

Fig. 3: Regulatory signature of CD8+ T cells stimulated via TCR without CD28 costimulation.

From: CD28 engagement inhibits CD73-mediated regulatory activity of CD8+ T cells

Fig. 3

Naïve CD8+ T cells (3 × 106/mL) from spleens of CD28KO (black) and CD28WT (red) mice were sorted and stimulated with anti-CD3/28 antibodies. On days 0, 2, 4, and 6 after stimulation, cells were harvested and subjected to flow cytometric analysis for the expressions of CD73, CD39, Foxp3, GITR, CTLA-4, LAG3, PD-1, CCR6, CD25, CD101, CD122, FR4, Gelactin-9, Helios and ICOS. The data presented are representative of one of the three independent experiments.

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