Fig. 4: Seeded cells secrete high levels of Aβ and α-syn aggregates.
From: Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells
Immunodepletion of Aβ and α-syn from culture medium was carried out using 6E10 or Syn211 antibody. The immunodepleted media were then imaged with AD-PAINT. a Number of aggregates per µm2 after immunodepletion. b Percentage of aggregates >75 nm in the detected population. c Numbers of aggregates removed by depletion with antibodies against Aβ and α-syn at defined time points. The numbers were calculated using the mean values of medium only controls and immunodepleted media as shown in a. Secreted aggregate of both Aβ and α-syn accumulate over time.
