Fig. 3: AGO2 close-ended cellular structures are cytokinetic protrusions.

Representative immunofluorescence images of NTHY ori 3-1 cells depicting the subcellular localization of AGO2 (green) with a Citron kinase (CITK) (red) and c Aurora B (red). Cell nuclei were stained blue (DAPI). b, d iPLA assays depicted the proximity of b AGO2–CITK and d AGO2–Aurora B as indicated by red foci. The black arrowheads in the Brightfield (BF) images indicate the midbody ring. The scale bar is 20 μm. Visual assessment (merged images, scatterplots, and intensity lineplots) of colocalization between paired proteins included in the yellow region of interest of the merged images. f, h AGO2 (green) with CITK and Dicer (red), respectively. e, g Aurora B in green with AGO2 and CITK in red, respectively. Lineplots indicate the signal intensity of the paired proteins along the lines overlaid on the corresponding images that appear in Supplementary Fig. S4d–g. The colocalization metrics (Pearson [R] and Manders’ [M1 and M2] coefficients) and respective p values are presented in Supplementary Table S1 (n = 6 biologically independent samples). Scale bar: 5 μm.