Fig. 4: AGO2 colocalizes with α-Tubulin in mitotic protrusions.

a Representative immunofluorescence images of NTHY ori 3-1 cells depicting the colocalization of AGO2 (green) with α-Tubulin (red). Cell nuclei were stained blue (DAPI). b iPLA assays demonstrated the high proximity of AGO2 and α-Tubulin by the presence of red foci. The black arrowhead in the BF images indicates the midbody ring. The scale bar is 20 μm. c–e Visual assessment (merged images, scatterplots, and intensity lineplots) of colocalization. Lineplots indicate the signal intensity of the paired proteins along the lines overlaid on the corresponding images that appear in Supplementary Fig. S4i–k. The colocalization metrics (Pearson [R] and Manders’ [M1 and M2] coefficients) and respective p values are presented in Supplementary Table S1. c, d AGO2 in green, α-Tubulin, and F-Actin (Texas red-phalloidin) in red. e α-Tubulin in green, F-Actin in red (n = 6 biologically independent samples). f Diagrammatic representation of the components of the cytokinetic intercellular bridge including AGO2, Dicer, CITK, Aurora B, α-Tubulin, and F-Actin. Scale bar: 5 μm.