Fig. 8: Activated kinases follow the AGO2/Dicer distribution in the midbody structure.

Representative images of NTHY ori 3-1 cells. AGO2 colocalization with a biotinylated oligo dT probe (scale bar: 20 μm). Small kinome analysis of b AGO2-phospho-MEK (scale bar: 10 μm), c AGO2-phospho-ERK (scale bar: 20 μm), d AGO2-phospho-p38 (scale bar: 20 μm), e AGO2-phospho-JNK (scale bar: 10 μm). a–e AGO2 proteins were visualized in green, οligo dT probe, phospho-MEK, phospho-ERK, phospho-p38, and phospho-JNK were visualized in red. b–e Visual assessment (merged images, scatterplots, and intensity lineplots) of colocalization. b–e Lineplots indicate the signal intensity of the two paired proteins. Images of AGO2 colocalization with f phospho-AGO2 Ser³⁸⁷ and phospho-AGO2 Ser³⁸⁷ with α-Tubulin in g untreated and h treated cells with SB205380 p38 inhibitor. f–h Phospho-AGO2 Ser³⁸⁷ in red, f AGO2 and g, h α-Tubulin in green, respectively. Nuclei were visualized in blue (DAPI). Scale bar: 20 μm.