Fig. 9: ΑGO2 is a stress-sensitive molecule.

Electron microscopy images of the stages of cell division of NTHY ori 3-1 cells depicting the cytokinetic structures up to abscission. a, b, f–h Section transmission electron micrographs and c–e scanning electron micrographs of dividing cells. a Dense bundled microtubules (black arrowhead) in the midzone and b a magnified midzone. c, d The midbody core and the electron-dense material (flemming body) concentrated at the midzone. e Cargoes are transferred through the microtubule protrusions. f, g A discontinued intercellular bridge between two daughter cells after abscission. The nucleus of the left-hand cell is visible. g A magnified abscission point. h The characteristic structural architecture of microtubular axoneme after the completion of abscission (black arrowhead). Membrane deposition is pictured by the black arrowhead. Scale bars: a 2 μm, b 500 nm, c 10 μm, d 5 μm, e 2 μm, f 2 μm, g 500 nm, and h 500 nm. i–l Representative images of NTHY ori 3-1 cells before and after heat shock. AGO2, Upf1, and TIAR subcellular distribution in i, k control and j, l heat-shocked cells. AGO2 is represented in green, Upf1, TIAR in red, and nuclei in DAPI (blue). The white arrowheads indicate cytokinetic events (scale bar: 20 μm).