Fig. 3: Analysis of heptapeptide aggregation.
From: The lexicon of antimicrobial peptides: a complete set of arginine and tryptophan sequences
a Analysis of the size of aggregates for peptides in stock solutions (800 µM) using DLS, indicating the three size categories identified (featuring small, moderate or large aggregates; n = 47, 72 and 10, respectively). b Box plots indicating ODvis distributions (i.e. likely overall extent of aggregation) for peptides in each aggregation state. Error bars shown are ±s.e.m. (n = 4). Numbers on brackets indicate the P value from a post hoc Tukey test of the aggregate states having the same distribution. c Box plots showing percentage W (by number of W residues in sequence) content distributions for peptides in each aggregation state. Numbers on brackets indicate the P value from a post hoc Tukey test of the aggregate states having the same distribution. d Antimicrobial activity (IC50) of peptides in each aggregation state for three test organisms. Numbers on brackets indicate the P value from a post hoc Tukey test of the aggregate states having the same distribution. e Haemolytic activity (EC50) for peptides in each aggregation state. Numbers on brackets indicate the P value from a post hoc Tukey test of the aggregate states having the same distribution. (n = 254 peptides in a–e). f Analysis of the size of aggregates present for three different peptides found to produce substantial aggregates at 800 µM after a tenfold dilution (i.e. to 80 µM) and using AUC. g TEM imaging of the peptide WRWWWWW at concentrations of 80 and 800 µM. Scale bars shown are 100 nm in length.
