Fig. 4: Method performance can be attributed to signal detection.
From: Comparison of bias and resolvability in single-cell and single-transcript methods
a Pairwise comparisons of methods that share the same steps for sample preparation and measurand, but differ in signal detection (indicated by “D” in the matrix), are used to attribute measurement performance to signal detection. The four boxes containing “D” under the matrix represent these same pairwise comparisons. b Pairwise AUC plots of measurements in (a) to compare resolvability between flow cytometry and microscopy. Diagonal line indicates equivalent resolvability between the two methods. Pairs of adjacent IPTG concentrations are shown as numbers within the plots, as indicated in the figure legend. Color indicates biological replicates one (orange), two (green), and three (purple), as indicated in the figure legend. Large gray numbers in the top-left and bottom right-corners indicate how many AUC’s were higher for the method plotted on the y-axis or x-axis, respectively. The two-sided p-values for a sign test are shown within each plot.
