Fig. 5: Method performance can be attributed to sample preparation.
From: Comparison of bias and resolvability in single-cell and single-transcript methods
a Pairwise comparisons of methods that share the same steps for signal detection and measurand, but differ in sample preparation (indicated by “P” in the matrix), are used to attribute measurement performance to sample preparation. The four boxes containing “P” under the matrix represent these same pairwise comparisons. b Pairwise AUC plots of measurements in (a) are used to compare resolvability between sample preparation methods (FISH versus HCR). Diagonal line indicates equivalent resolvability between the two methods. Pairs of adjacent IPTG concentrations are shown as numbers within the plots, as indicated in the figure legend. Color indicates biological replicates one (orange), two (green), and three (purple), as indicated in the figure legend. Large gray numbers in the top-left and bottom right-corners indicate how many AUC’s were higher for the method plotted on the y-axis or x-axis, respectively. The two-sided p-values for a sign test are shown within each plot.
