Fig. 5: T cell-related function profiles of regenerated TIL-iPS-T.

a Proliferation capacity of TI-CTL and TIL-iPS-T reacting to the PHA/PBMC method. *P < 0.05, **P < 0.01, ****P < 0.0001, two-tailed paired t test. Dots represent data from individual experiments. Means are shown; error bars represent SD. b Cytokine production capacity was evaluated for C-T-10 MMR-D. Representative data of three independent experiments are shown. Left: data were acquired by co-culturing with cancer spheroids and gated on CD45⁺ cells. Right: data were acquired by stimulating with PMA plus ION and gated on CD45⁺CD4⁻CD8α⁺CD8β⁺ cells. c, d Killing function of tumor-specific clones was quantified by the luminescence-based killing assay. Data from MMR-D (c) and MMR-P (d). Representative data of four independent experiments are shown. Dots represent individual values. Means are connected by lines; error bars represent SD. n = 3 per point. e Killing function of tumor nonspecific clones was quantified by the luminescence-based killing assay. Representative data of four independent experiments are shown for C-T-10 Vβ23 and seven independent experiments are shown for C-T-7 Vβ5.3. Dots represent individual values. Means are connected by lines; error bars represent SD. n = 3 per point.