Fig. 6: Persistency-related profiles of regenerated TIL-iPS-T. | Communications Biology

Fig. 6: Persistency-related profiles of regenerated TIL-iPS-T.

From: The therapeutic potential of multiclonal tumoricidal T cells derived from tumor infiltrating lymphocyte-derived iPS cells

Fig. 6

a Bar graphs depict the relative telomere length (RTL) of TI-CTL and TIL-iPS-T. *P < 0.05, ***P < 0.001, two-tailed unpaired t test. Dots represent data from individual experiments. Means are shown; error bars represent SD. b Representative mitochondria function analysis from two independent experiments are shown. Left: Bar graphs depict the SRC of TI-CTL and TIL-iPS-T. *P < 0.05, **P < 0.01, ****P < 0.0001, NS, not significant, two-tailed unpaired t test. Dots represent individual values. Means are shown; error bars represent SD. Right: Oxygen consumption rate (OCR) trace data for C-T-10 Vβ2 are shown. Means are shown with dots and connected by lines; error bars represent SD. c Cell division capacity of TI-CTL and TIL-iPS-T were assessed by the CFSE-dilution assay. CFSE-labeled cells were co-cultured with cancer spheroids for 7 days. Representative data of two independent experiments are shown. Data were gated on CD45+ live cells. d Flow cytometry data about apoptosis sensitivity are indicated. Both TI-CTL and TIL-iPS-T were co-cultured with autologous cancer spheroids for 48 h. Representative data of three independent experiments are shown. Data were gated on CD45+ cells.

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