Fig. 7: In vivo dynamics of TIL-iPS-T. | Communications Biology

Fig. 7: In vivo dynamics of TIL-iPS-T.

From: The therapeutic potential of multiclonal tumoricidal T cells derived from tumor infiltrating lymphocyte-derived iPS cells

Fig. 7

a A schema of the experiment design. Non-labeled cancer spheroids were used for the PDSX establishment. In total, 7.5 × 106 firefly luciferase-transduced (FF-Luc+) TI-CTL or TIL-iPS-T were intravenously injected into PDSX mice. Cytokines (IL-2 200 U/mouse, IL-7 50 ng/mouse, IL-15 50 ng/mouse) were intraperitoneally injected three times a week. b The percentages of human CD45+ cells within the mouse PBMC fraction are indicated. Dots represent individual values. Means are connected by lines; error bars represent SD. n = 3 per point except day 21 (n = 2). *P < 0.05, two-tailed unpaired t test. c Bioluminescence images by an in vivo imaging system (IVIS) are shown. Bioluminescence signals were captured from FF-Luc+ TI-CTL or TIL-iPS-T. d Histological images of an isolated tumor on day 14 are shown. Upper: hematoxylin eosin (HE) staining. Lower: immunostaining of human CD8. Arrows indicate TI-CTL or TIL-iPS-T stained with CD8 antibody. Scale bars indicate 50 µm.

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