Fig. 7: Power loss due to segregation distortion.
a Effect of segregation distortion on mapping power with NF54 × NHP4026 progeny with simulated phenotype data at different ES. Each sub-panel shows the relationship between allele frequency and power for different numbers of progeny at a fixed ES. For a high ES, allele frequency has little effect on power. At lower ES, we observe a large loss of power for alleles with less than 0.3 allele frequency. b Distribution of mean ± standard deviation of the chloroquine IC50 from five biological replicates of 56 progeny and two parents from the NF54 × NHP4026 cross. c QTL mapping of mean chloroquine IC50 (ES = 0.84) in (b) results in a LOD score of 31.13 and a genome-wide p value of 0.0007 demonstrating that despite extreme segregation distortion, QTL can be detected for experimental phenotype data with high ES.
