Fig. 2: Oligomerization of HER2 (~180 kDa) by bipDARPins and antibodies on BT474 cells analyzed by chemical crosslinking and two-color western blot. | Communications Biology

Fig. 2: Oligomerization of HER2 (~180 kDa) by bipDARPins and antibodies on BT474 cells analyzed by chemical crosslinking and two-color western blot.

From: Apoptosis-inducing anti-HER2 agents operate through oligomerization-induced receptor immobilization

Fig. 2

a Comparison of the size of HER2 complexes after treatment with the bipDARPin 6L1G (32 kDa) with those obtained with growth factor heregulin (HRG, 27 kDa), monovalent DARPins (9.26 and G3, ~12 kDa each) or a bivalent, monoparatopic DARPin (GL4G, ~30 kDa). Primary antibodies against HER2 and DARPins were used. b Crosslinked HER2 complexes upon treatment with antibodies trastuzumab (TZB, ~145 kDa) and pertuzumab (PZB, ~145 kDa), detected by primary antibodies against HER2 and human IgG.

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