Fig. 3: Dependence of the maximal rates L-Asp transport rate on the concentrations of Na⁺ and L-aspartate.

a Dependence of the maximal rates of transport \({v}_{max}^{\mathrm{Na}}\) (app) from Fig. 1a on the concentration of L-Asp. b Dependence of the maximal rates of transport \({v}_{max}^{\mathrm{Asp}}\) (app) from Fig. 1b on the concentration of sodium ions. Solid and dashed lines represent fits of rectangular hyperbolic functions to the data and the 95% confidence intervals, respectively. In panel A the fitted value of k_cat/k₃ (Eq. (13)) is indicated. Each value represents the average of three independent biological replicates, and two technical replicates. The standard error of the mean is shown. The data represent the combined contributions of right-side-out and inside-out oriented proteins.