Table 1 Initial rates of L-aspartate uptake by GltTk reconstituted in proteoliposomes. In the first column, the concentrations in parentheses indicate the amount of choline chloride used in the external reaction buffer to balance the osmotic and ionic strength. Each uptake rate represents the average of three independent biological replicates and independent reconstitutions, each with two technical replicates. The standard error of the mean is indicated.
From: Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
[L-Asp]ā [Na+ā] | 0.05āµM | 0.1āµM | 0.5āµM | 1āµM | 5āµM | 10āµM | 50āµM | 100āµM |
---|---|---|---|---|---|---|---|---|
1āmM (299āmM) | Not determined | Not determined | Not determined | 0.03 ±ā2.6 10ā2āminā1 | 0.51 ±ā0.3āminā1 | 0.25 ±ā0.3āminā1 | 2.85 ±ā1.2āminā1 | 10.93 ±ā4.4āminā1 |
5āmM (295āmM) | 0.05 ±ā1.6 10ā2āminā1 | 0.06 ±ā2.1 10ā2āminā1 | 0.59 ±ā0.1āminā1 | 0.96 ±ā0.2āminā1 | 3.62 ±ā0.7āminā1 | 6.48 ±ā0.9āminā1 | 16.59 ±ā2.8āminā1 | 21.62 ±ā3.2āminā1 |
10āmM (290āmM) | 0.21 ±ā5.5 10ā2āminā1 | 0.28 ±ā0.1āminā1 | 2.16 ±ā0.4āminā1 | 3.75 ±ā0.6āminā1 | 8.19 ±ā1.2āminā1 | 12.60 ±ā1.9āminā1 | 25.08 ±ā5.0āminā1 | 30.51 ±ā4.1āminā1 |
25āmM (275āmM) | 0.95 ±ā0.2āminā1 | 1.46 ±ā0.5āminā1 | 8.53 ±ā1.2āminā1 | 11.56 ±ā1.7āminā1 | 19.09 ±ā2.0āminā1 | 26.39 ±ā3.0āminā1 | 40.58 ±ā6.4āminā1 | 43.59 ±ā6.9āminā1 |
50āmM (250āmM) | 1.88 ±ā0.4āminā1 | 3.34 ±ā1.0āminā1 | 16.79 ±ā2.6āminā1 | 21.26 ±ā2.3āminā1 | 31.28 ±ā3.5āminā1 | 39.30 ±ā4.9āminā1 | 43.69 ±ā8.3āminā1 | 50.21 ±ā4.4āminā1 |
100āmM (200āmM) | 2.75 ±ā0.5āminā1 | 4.79 ±ā1.5āminā1 | 23.46 ±ā2.5āminā1 | 30.47 ±ā3.3āminā1 | 38.90 ±ā3.5āminā1 | 52.16 ±ā7.7āminā1 | 53.52 ±ā7.9āminā1 | 68.13 ±ā3.5āminā1 |
200āmM (100āmM) | 3.06 ±ā0.6āminā1 | 4.83 ±ā1.5āminā1 | 31.27 ±ā6.4āminā1 | 32.55 ±ā3.8āminā1 | 37.29 ±ā4.0āminā1 | 49.80 ±ā9.0āminā1 | 51.90 ±ā6.7āminā1 | 65.40 ±ā7.5āminā1 |
300āmM (0āmM) | 2.93 ±ā0.7āminā1 | 4.96 ±ā1.6āminā1 | 27.72 ±ā5.3āminā1 | 28.95 ±ā4.0āminā1 | 40.12 ±ā5.4āminā1 | 46.98 ±ā7.9āminā1 | 52.69 ±ā8.5āminā1 | 61.77 ±ā2.9āminā1 |