Table 5 Cryo-EM data collection, refinement, and validation.
From: Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
Data collection and processing | |
āVoltage (kV) | 200 |
āElectron exposure (eā/Ć 2) | 53.3 |
āDefocus range (Ī¼m) | ā0.5 to ā2.0 |
āPixel size (Ć ) | 1.012 |
āSymmetry imposed | C1 |
āInitial dataset (# of particles) | 109217 |
āFinal dataset (# of particles) | 53983 |
āMap resolution (Ć ) FSC0.143 | 3.5 |
Refinement | |
āInitial model used | PDB 6XWQ |
Model composition | |
āNonhydrogen atoms | 10357 |
āProtein residues | 1378 |
āLigands | 3 |
Mean B factors (Ć 2) | |
āProtein | 160.2 |
āLigand | 165.8 |
Rms deviations | |
āBond lengths (Ć ) | 0.005 |
āBond angles (Ā°) | 0.635 |
Validation | |
āMolProbity score | 2.09 |
āClash score | 17.7 |
āPoor rotamers (%) | 0.00 |
āRamachandran plot (%) | |
āāFavored | 95.07 |
āāAllowed | 4.93 |
āāOutliers | 0.00 |
āāModel to map fit CC | 0.86 |
