Fig. 2: Non-cognate Gɑ couplings at the AM1 receptor complex modulates cAMP accumulation and ERK1/2 phosphorylation. | Communications Biology

Fig. 2: Non-cognate Gɑ couplings at the AM1 receptor complex modulates cAMP accumulation and ERK1/2 phosphorylation.

From: CGRP, adrenomedullin and adrenomedullin 2 display endogenous GPCR agonist bias in primary human cardiovascular cells

Fig. 2

a Characterisation of cAMP accumulation in response to stimulation by CGRP, AM, and AM2 with and without PTX treatment relative to forskolin (100 μM) (n = 4). b–e Characterisation of ERK1/2 phosphorylation in response to stimulation by CGRP, AM, and AM2 with and without PTX treatment relative to PMA (10 μM) (n = 4) (b), with/without Rp-8-Br-cAMPS (10 μM) (n = 3) (c), with/without YM-254890 (100 nM) relative to PMA (10 μM) (n = 3) (d) and with/without ESI-09 (100 μM) (n = 3) (e). Data are analysed using a three-parameter non-linear regression curve. All data represent mean ± SEM of n independent experiments. Statistical significance determined compared to control using an unpaired Student’s t test with Welch’s correction (*p < 0.05; **p < 0.01; ***p < 0.001). NS denotes no statistical significance observed. Rows show pEC50, and vertical arrows show Emax statistical significance.

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