Fig. 5: LPA-PKD-1 signaling in breast cancer stemness. | Communications Biology

Fig. 5: LPA-PKD-1 signaling in breast cancer stemness.

From: Development of an arteriolar niche and self-renewal of breast cancer stem cells by lysophosphatidic acid/protein kinase D signaling

Fig. 5

a Tumorsphere formation of mouse BC cells. CD44+ E0771 cells were cultured in complete MammoCult™ Medium with the treatment of 10 µm LPA, 1 µm CRT0066101 (PKD inhibitor), 1 µm Ki16425 or their combination for 7 days. b Tumor sphere formation of ER+BC cells. Human ER+BC cells (MCF-7) were cultured in complete MammoCult™ Medium with the treatment of 10 µm LPA, 1 µm CRT0066101 (PKD inhibitor), 1 µm Ki16425 or their combination for 7 days. The mammary spheres were counted under the OLYMPUS CK30 microscope, triplicate experiments were performed, and the results are shown as the mean value \(\pm\) SEM. ***P < 0.001 compared with control or LPA treatment. c LPA-PKD-1 signaling stimulated the expression of Oct4 in ER+BC cells. **P < 0.01, compared with control or LPA treatment. d LPA-PKD-1 signaling stimulated the expression of KLF4 in ER+BC cells. ***P < 0.001, compared with control or LPA treatment. e Sox2 expression was regulated differently by LPA/PKD-1 signaling. Total RNA was extracted from MCF-7 tumorspheres with different treatments, and mRNA levels were assayed with RT-qPCR. The results of triplicate experiments are shown as mean ± SEM. ***P < 0.001 or #P < 0.001 compared with the control or LPA treatment.

Back to article page