Fig. 6: PKD-1 is required for tumorigenicity by tumorsphere formation efficiency assay. | Communications Biology

Fig. 6: PKD-1 is required for tumorigenicity by tumorsphere formation efficiency assay.

From: Development of an arteriolar niche and self-renewal of breast cancer stem cells by lysophosphatidic acid/protein kinase D signaling

Fig. 6

a Immunoblotting assay indicates that the PKD-1 expression decreased when transfecting MCF-7 with PKD-1 siRNA as compared with the scramble control (CTL). b MCF-7 with PKD-1 depletion significantly reduced tumorsphere formation efficiency. c Quantitation graph shows that the number of tumorspheres formed in each well with the seeding density starting from 500 to 20 cells/well by statistical analysis. d Representative images for tumorsphere formation in MCF-7 cells subjected to siRNA scramble control (CTL) and PKD-1 siRNA and with different seeding densities.

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