Fig. 4: Differential receptor binding by anti-CD40 hIgG1 and hIgG2.
From: TNF receptor agonists induce distinct receptor clusters to mediate differential agonistic activity
a Recombinant hCD40ECD was immobilised onto CM5 chip via amine coupling and various anti-CD40 mAbs as h1 or h2 were injected at 250, 50, 10, 2, 0.4, and 0 nM using a Biacore T200 instrument. Both association phase and dissociation phase lasted 300 s. KD values were calculated using Biacore Bioevaluation software and values represent the mean from three independent experiments. b Ramos cells were incubated with various anti-CD40 mAbs as h1 or h2 as indicated and then washed and bound hIgG was detected by polyclonal anti-human IgG Fc, c monoclonal anti-human kappa light chain (left panel histograms represent cell surface binding of 341G2 h1 or h2 at 20 µg/mL) or d monoclonal anti-hIgG Fc. Means ± SEM, n = 3, data representative of independent 3 experiments.
