Fig. 4: The MUFA metabolism plays a crucial role in the regulation of the type I IFN response and antiviral response in CD4⁺ T cells.

a qRT-PCR analyses of the relative expression of ISGs in Th1 cells treated with 10 μM TOFA, 1 μM Mk-8245, or 30 μM Sc-26196. Relative expression (normalized to Hprt) with SD is shown. b qRT-PCR analyses of the relative expression of ISGs in control, sgScd2 and sgFads2 Th1 cells. Relative expression (normalized to Hprt) with SD is shown. c MLE-15 cells were cultured with culture supernatants of control, sgScd2 and sgFads2 Th1 cells and infected with x31. Virus titers were determined 72 h p.i. (n = 3 per each group biologically independent sample). d The amount of IFNα in the cell supernatant was measured by ELISA. Data are means ± SD. e, f qRT-PCR analyses of relative expression of ISGs in control, sgScd2 Th1 cells supplemented with 30 μM palmitic acid, 10 μM stearic acid, 30 μM oleic acid (e) or 10 μg/ml cholesterol (f) for 72 h. g, h Survival rate (g) or weight change (h) of PR8 infected mice was assessed 1 day after administrative transfer of control or sgScd2 Th1 cells. Relative expression (normalized to Hprt) with SD is shown. More than two independent experiments were performed and showed similar results (a–f = 3). Three technical replicates were performed with quantitative RT-PCR and relative expression (normalized to Hprt) with SD is shown (a, b, e, f).