Fig. 6: OA-containing TG is essential for the activation of type I IFN response in CD4+ T cells. | Communications Biology

Fig. 6: OA-containing TG is essential for the activation of type I IFN response in CD4+ T cells.

From: SCD2-mediated monounsaturated fatty acid metabolism regulates cGAS-STING-dependent type I IFN responses in CD4+ T cells

Fig. 6

a Microscopy analysis was performed using control and sgScd2 Th1 cells stained with lipid droplet, cytosol, and nuclear. b Intracellular staining and flow cytometry analyzing of lipid droplet in control and sgScd2 Th1 cells. Oleic acid was treated for 72 h in sgScd2 Th1 cells. Mean fluorescence intensity (MFI) of lipid droplet are shown. c Summary data of four independent experiments of lipid droplet contents are shown. Each dot represents one experiment. Data are means ± SD. d Pie chart showed that the ratio of lipid species control Th1 cells detected by lipidomics analysis. e A heat map depicts the lipid relevant to (d). f The lipidomics analysis reveals the relative contents of molecular lipid species in ACC1−/−, sgScd2, and sgFads2 Th1 cells compared to control Th1 cells. Normalized values were shown here. Two independent samples were conducted for lipidomics analysis. (n = 2 per each group biologically independent sample). g Pie chart showed that the ratio of fatty acid species incorporated into lipid detected by lipidomics analysis. h Species of lipid contents with or without oleic acid were shown. i A heat map depicts the relative contents of TGs. j A scatter plot depicts lipid contents of control and sgScd2 Th1 cells. The dashed lines indicate 50% changed line for the difference in lipid contents. The X-axis shows the mean value of control and sgScd2 Th1 cells, and the Y-axis shows the percentage of sgScd2 Th1 cells relative to control. k qRT-PCR analyses of the relative expression of ISGs in control, sgScd2, and sgGpam Th1 cells. Relative expression (normalized to Hprt) with SD is shown. l qRT-PCR analyses of the relative expression of ISGs in control, sgScd2, and sgDgat1 Th1 cells. Relative expression (normalized to Hprt) with SD is shown. Two biological replicates were performed for lipidomics analysis (d–j). More than three independent experiments were performed and showed similar results (a, b, c, k, l). Three technical replicates were performed with quantitative RT-PCR and relative expression (normalized to Hprt) with SD is shown in RT-PCR (k, l).

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