Fig. 2: Flp:shRNA parameter space highlights divergent performance of each topology.

AUC (A), SNR (B), and fold change (C) heat maps highlight trends among each circuit topology. Outlined sections in the AUC and SNR heat maps represent topologies achieving AUC > 0.95 and SNR > 0 dB, respectively. Data represents the average of three technical replicates (n = 3) of circuits transiently transfected into HEK293FT cells 48 h after transfection/induction. Induced cell media contains 225 nM dox.